MHC class II (I-A/I-E) Antibody (M5/114.15.2)

Product Discontinued

MHC class II (I-A/I-E) Antibody (M5/114.15.2) Summary

• Immunogen: Activated C57BL/6 mouse spleen cells
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: H2-Ea
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse, Reacts with a shared determinant on I-Ab,d,q, and I-Ed,k alloantigens..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2)

• E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea
• H2-Ea
• MHC class II (I-A/I-E)
• MHC class II IA/IE

Background

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2p,r haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MHC class II (I-A/I-E) Antibody (NB100-77508)(2)

Publications using NB100-77508

• Fabrik I, Link M, Hartlova A et al. Application of SILAC Labeling to Primary Bone Marrow-Derived Dendritic Cells Reveals Extensive GM-CSF-Dependent Arginine Metabolism J Proteome Res (FLOW, Mouse)
  
  Details:
  This publication used the Biotin conjugated form of this antibody (Cat# NB100-77509).
• Fabrik I, Link M, Hartlova A et al. Application of SILAC Labeling to Primary Bone Marrow-Derived Dendritic Cells Reveals Extensive GM-CSF-Dependent Arginine Metabolism J Proteome Res 2013-12-07 [PMID: 24308431]

Bioinformatics

• Gene Symbol: H2-Ea