MHC Class I Antibody (14-4-4S)


Flow Cytometry: MHC Class I Antibody (14-4-4S) [NBP1-43736] - Analysis using the Biotin conjugate of NBP1-43736. Staining of mouse splenocytes with Anti-Mouse/Rat MHC Class II (I-Ek) FITC (left) or PE (right). more

Product Details

Reactivity Mu, RtSpecies Glossary
Applications Flow
0.5 mg/ml

MHC Class I Antibody (14-4-4S) Summary

The immunogen for this antibody was PE MHC class II.
IgG2a Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
Application Notes
The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at less than or equal to 0.06 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reactivity Notes

This antibody is reactive to Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for MHC Class I Antibody (14-4-4S)

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1


The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2d, H-2p and H-2r. H-2b, H-2f and H-2s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43736AF405
Alexa Fluor 488 Labeled NBP1-43736AF488
Alexa Fluor 647 Labeled NBP1-43736AF647
Alexa Fluor 700 Labeled NBP1-43736AF700
Allophycocyanin Labeled NBP1-43736APC
DyLight 350 Labeled NBP1-43736UV
DyLight 405 Labeled NBP1-43736V
DyLight 405LS Labeled NBP1-43736V3
DyLight 488 Labeled NBP1-43736G
DyLight 680 Labeled NBP1-43736FR
DyLight 755 Labeled NBP1-43736IR
PE Labeled NBP1-43736PE
PerCP Labeled NBP1-43736PCP

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Contact Information

Product PDFs


Gene Symbol HLA-E

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