mGluR7 Antibody (7G8D3) - BSA Free

Western Blot: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis using GRM7 mAb against human GRM7 (AA: 454-590) recombinant protein. (Expected MW is 41.7 kDa)

Immunocytochemistry/Immunofluorescence: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis of SMMC-7721 cells using GRM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Western Blot: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis using GRM7 mAb against HEK293 (1) and GRM7 (AA: 454-590)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis of Hela cells using GRM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

ELISA: mGluR7 Antibody (7G8D3) [NBP2-61856] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis of SH-SY5Y cells using GRM7 mouse mAb (green) and negative control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 7G8D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

mGluR7 Antibody (7G8D3) - BSA Free Summary

• Description: Novus Biologicals Mouse mGluR7 Antibody (7G8D3) - BSA Free (NBP2-61856) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human mGluR7 (AA: 454-590) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRM7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:100-1:2000
• Theoretical MW: 102.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for mGluR7 Antibody (7G8D3) - BSA Free

• FLJ40498
• glutamate receptor, metabotropic 7
• GPRC1G
• GRM7
• metabotropic glutamate receptor 7
• MGLU7
• mGluR7
• MGLUR7GPRC1GGLUR7

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRM7