Methionyl tRNA synthetase Antibody - BSA Free

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-33016] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MARS protein stained by MARS antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Methionyl tRNA synthetase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Methionyl tRNA synthetase Antibody - BSA Free (NBP1-33016) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Methionyl tRNA synthetase. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Monkey (97%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (97%), Xenopus laevis (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Methionyl tRNA synthetase Antibody - BSA Free

• cytosolic methionyl-tRNA synthetase
• EC 6.1.1.10
• FLJ35667
• methionine tRNA ligase 1, cytoplasmic
• Methionine--tRNA ligase
• methionyl-tRNA synthetase
• methionyl-tRNA synthetase, cytoplasmic
• MetRS
• MRS
• MTRNS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MARS1