Metadherin Antibody (CL0397) - BSA Free

Western Blot: LYRIC Antibody (CL0397) [NBP2-52929] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of U-251 cells using the Anti-MTDH monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human testis shows strong cytoplasmic positivity in seminiferous tubules.

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human breast cancer shows strong cytoplasmic immunoreactivity in cancer cells.

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human colorectal cancer shows cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human liver cancer shows granular cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human placenta shows absence of staining in the placental trophoblast (negative control).

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human prostate cancer shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL0397
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Metadherin Antibody (CL0397) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q86UE4, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (93%), Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MTDH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  Metadherin Recombinant Protein Antigen (NBP2-52929PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Metadherin Antibody (CL0397) - BSA Free

• 3D3
• AEG1
• AEG-13D3/LYRIC
• AEG1LYRIC/3D3
• Astrocyte elevated gene-1 protein
• LYRIC
• LYRICMetastasis adhesion protein
• Lysine-rich CEACAM1 co-isolated protein
• Metadherin
• MTDH
• protein LYRIC

Background

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumour tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MTDH