Metadherin Antibody (CL0397) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.

Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Staining of U-251 cells using the Anti-Metadherin monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0397
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Metadherin Antibody (CL0397) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q86UE4, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MTDH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Metadherin Antibody (CL0397) - Azide and BSA Free

• 3D3
• AEG1
• AEG-13D3/LYRIC
• AEG1LYRIC/3D3
• Astrocyte elevated gene-1 protein
• LYRIC
• LYRICMetastasis adhesion protein
• Lysine-rich CEACAM1 co-isolated protein
• Metadherin
• MTDH
• protein LYRIC

Background

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumour tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MTDH