Metadherin Antibody (2F11C3) - BSA Free

Western Blot: LYRIC Antibody (2F11C3) [NBP1-51585] - Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.

Immunohistochemistry-Paraffin: LYRIC Antibody (2F11C3) [NBP1-51585] - Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb

Flow Cytometry: LYRIC Antibody (2F11C3) [NBP1-51585] - Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 2F11C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Metadherin Antibody (2F11C3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MTDH
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% glycerol
• Preservative: 50% glycerol
• Purity: Protein G purified

Alternate Names for Metadherin Antibody (2F11C3) - BSA Free

• 3D3
• AEG1
• AEG-13D3/LYRIC
• AEG1LYRIC/3D3
• Astrocyte elevated gene-1 protein
• LYRIC
• LYRICMetastasis adhesion protein
• Lysine-rich CEACAM1 co-isolated protein
• Metadherin
• MTDH
• protein LYRIC

Background

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumor tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Metadherin Antibody (NBP1-51585)(2)

Publications using NBP1-51585

• Ahn S, Hyeon J, Park CK. Metadherin is a prognostic predictor of hepatocellular carcinoma after curative hepatectomy. Gut Liver 2013-03-01 [PMID: 23560157] (IHC-P, Human)
• Wang K, Lim HY, Shi S et al. Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma Hepatology 2013-03-18 [PMID: 23505090] (IHC-P, Human)

FAQs for Metadherin Antibody (NBP1-51585). (Showing 1 - 2 of 2 FAQs).

1. Could you give me some details about the NBP1-51585? Especially the epitope, I really want to know it exactly.
   • The exact immunogen sequence is proprietary, but the immunogen range is between 378-579. The antibody has not been epitope mapped to our knowledge.
2. I was just wondering whether the HeLa cells used in the flow cytometry were permeabilised or not? As I am interested in looking whether LYRIC is surface expressed on my cell type.
   • The HeLa cells were permeabilised by using 0.1% Triton 100.

Bioinformatics

• Gene Symbol: MTDH