Mesp1 Antibody (1A3)

Western Blot: Mesp1 Antibody (1A3) [NBP1-51613] - Western blot analysis using MESP1 mAb against MESP1-hIgGFc transfected HEK293 cell lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Mesp1 Antibody (1A3) Summary

• Description: Novus Biologicals Mouse Mesp1 Antibody (1A3) (NBP1-51613) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Mesp1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Mesp1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MESP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 18369102).
• Theoretical MW: 28.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18369102). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Mesp1 Antibody (1A3)

• bHLHc5
• bHLHc5MGC10676
• Class C basic helix-loop-helix protein 5
• mesoderm posterior 1 homolog (mouse)
• mesoderm posterior protein 1
• MESP1

Background

Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways (By similarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mesp1 Antibody (NBP1-51613)(3)

Publications using NBP1-51613

• Bansal A, Pandey MK, Yamada S et al. [89Zr]Zr-DBN labeled cardiopoietic stem cells proficient for heart failure Nuclear medicine and biology 2020-09-09 [PMID: 32957056] (ICC/IF, Mouse, Human)
• Seo HR, Joo HJ, Kim DH et al. Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement. ACS Appl Mater Interfaces 2017-05-24 [PMID: 28497946]
• Nelson TJ, Faustino RS, Chiriac A et al. CXCR4+/FLK-1+ biomarkers select a cardiopoietic lineage from embryonic stem cells. Stem Cells 2008-06-01 [PMID: 18369102] (ICC/IF, Mouse)

FAQs for Mesp1 Antibody (NBP1-51613). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you could provide the sequence of the recombinant fragment used to generate this antibody.
   • The exact immunogen sequence for our product NBP1-51613 is proprietary, but I can tell you that the immunogen falls between amino acids 1-200 of the human MESP1 protein.

Bioinformatics

• Gene Symbol: MESP1