Mesothelin Antibody

Western Blot: Mesothelin Antibody [NBP1-31445] - Sample(30 ug of whole cell lysate) A:293T 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-31445] - Paraffin-embedded serous OVCA, using antibody at 1:100 dilution.

Product Discontinued

Mesothelin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Mesothelin. The exact sequence is proprietary.
• Localization: Cell membrane, Lipid-anchor, GPI-anchor, Golgi apparatus, Megakaryocyte-potentiating factor: Secreted, Isoform 3: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Mesothelin Antibody

• CAK1 antigen
• CAK1
• megakaryocyte potentiating factor
• Mesothelin
• MPF
• MPFSMRP
• MSLN
• Pre-pro-megakaryocyte-potentiating factor
• SMR
• soluble MPF mesothelin related protein

Background

An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MSLN