Mesothelin Antibody (618922) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 618922
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

Mesothelin Antibody (618922) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human Mesothelin in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mesothelin Antibody (618922) [Alexa Fluor™ Plus 555]

• CAK1 antigen
• CAK1
• megakaryocyte potentiating factor
• Mesothelin
• MPF
• MPFSMRP
• MSLN
• Pre-pro-megakaryocyte-potentiating factor
• SMR
• soluble MPF mesothelin related protein

Background

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Alternate splicing generates additional Mesothelin isoforms that have either an eight amino acid insertion following Ser408 or a substituted C-terminal region with no GPI anchor (6). Within aa 29-580, human Mesothelin shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 7). It is upregulated in mesotheliomas and a range of carcinomas and adenomas (8-11). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (10, 12). It is coexpressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (11, 14, 15).

1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
6. Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
7. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
8. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
9. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
10. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
11. Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
12. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
13. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
14. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
15. Robinson, B.W.S. et al. (2003) Lancet 362:1612.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.