MESDC2 Antibody [Unconjugated]

Western blot shows lysates of mouse brain tissue, NS0 mouse myeloma cell line, and D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MESDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MESDC2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MESDC2 was detected in perfusion fixed frozen sections of mouse testis using 10 µg/mL Goat Anti-Mouse MESDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4545) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MESDC2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MESDC2
  Ala30-Leu224
  Accession # Q9ERE7
• Specificity: Detects mouse MESDC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with human MESDC2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MESDC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MESDC2 Antibody [Unconjugated]

• BOCA
• KIAA0081Renal carcinoma antigen NY-REN-61
• MESDC2
• MESDLDLR chaperone MESD
• mesoderm development candidate 2Mesoderm development protein

Background

Mesoderm development candidate gene 2 (MESDC2, Mesd), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis (1-3). Mature mouse MESDC2 consists of an 83 amino acid (aa) structured central domain with flexible N- and C-terminal regions (4, 5). It shares 89% and 96% aa sequence identity with human and rat MESDC2, respectively. Although Boca lacks 34 aa in the C-terminal region and is only 40% identical with mouse MESDC2, the mouse protein can functionally substitute for Boca in drosophila S2 cells (6). A C-terminal ER retention motif localizes MESDC2 and Boca to the lumen of the endoplasmic reticulum (2, 6, 7). Within the ER, MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 and is required for their proper folding and cell surface expression (2, 5, 8, 9). MESDC2 is therefore important for cellular Wnt responsiveness (9). When added extracellularly, MESDC2 binds to cell surface LRP6, preventing its interaction with the Wnt antagonist Dkk-1. This binding does not, however, trigger LRP6 internalization or alteration of cytoplasmic beta -catenin levels (8). An LRP5 mutant associated with high bone mass does not interact with MESDC2 (10). MESDC2 itself can be disrupted by a chromosomal translocation occurring in the germ cell tumor, infantile sacrococcygeal teratoma (11).

1. Holdener, B.C. et al. (1994) Development 120:1335.
2. Hsieh, J-C. et al. (2003) Cell 112:355.
3. Kimelman, D. (2006) Nat. Rev. Genet. 7:360.
4. Wines, M.E. et al. (2001) Genomics 72:88.
5. Koduri, V. and S.C. Blacklow (2007) Biochemistry 46:6570.
6. Culi, J. et al. (2004) EMBO J. 23:1372.
7. Culi, J. and R.S. Mann (2003) Cell 112:343.
8. Li, Y. et al. (2005) J. Cell Sci. 118:5305.
9. Li, Y. et al. (2006) FEBS Lett. 580:5423.
10. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
11. Veltman, I.M. et al. (2005) Hum. Mol. Genet. 14:1955.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MESDC2
• Uniprot:
  • Q9ERE7()