Meprin beta Subunit/MEP1B Antibody (289731) [Unconjugated]

Western blot shows lysates of human intestine tissue and mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Meprin beta Subunit/MEP1B was detected in immersion fixed paraffin-embedded sections of human intestine using Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to the Brush border of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 289731
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Meprin beta Subunit/MEP1B Antibody (289731) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B
  Thr23-Ser293
  Accession # Q16820
• Specificity: Detects the pro form of human or mouse Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: MEP1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin beta Subunit/MEP1B Antibody (289731) [Unconjugated]

• EC 3.4.24.63
• endopeptidase-2
• MEP1B
• meprin A subunit beta
• meprin A, beta
• Meprin B
• Meprin beta Subunit
• N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
• PABA peptide hydrolase
• PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only) (3). Although the two subunits share 42% amino acid (aa) sequence identity, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of human Merpin beta subunit precursor consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 61), and a mature chain (aa 62 to 701) containing catalytic (aa 62 to 259), MAM (aa 260 to 429), MATH (aa 430 to 585), EGF-like (aa 604 to 644), transmembrane (aa 653 to 673), and cytoplasmic (aa 674 to 701) domains.

1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
2. Stocker, W. et al. (1995) Protein Sci. 4:823.
3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meprin beta Subunit/MEP1B Antibody (MAB28951)(4)

Publications using MAB28951

• Linjun Hong, Xupeng Zang, Qun Hu, Yanjuan He, Zhiqian Xu, Yanshe Xie et al. Uterine luminal-derived extracellular vesicles: potential nanomaterials to improve embryo implantation Journal of Nanobiotechnology 2023-03-07 [PMID: 36882792]
• Daniel Kruppa, Florian Peters, Olivier Bornert, Mareike D. Maler, Stefan F. Martin, Christoph Becker-Pauly et al. Distinct contributions of meprins to skin regeneration after injury – Meprin alpha a physiological processer of pro-collagen VII Matrix Biology Plus 2021-08-01 [PMID: 34435182]
• Tomas J, Mulet C, Saffarian A et al. High-fat diet modifies the PPAR-gamma pathway leading to disruption of microbial and physiological ecosystem in murine small intestine Proc. Natl. Acad. Sci. U.S.A. 2016-09-16 [PMID: 27638207]
• Michael J. Randles, Adrian S. Woolf, Jennifer L. Huang, Adam Byron, Jonathan D. Humphries, Karen L. Price et al. Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization Journal of the American Society of Nephrology 2015-12-01 [PMID: 25896609]

Bioinformatics

• Gene Symbol: MEP1B
• Uniprot:
  • Q16820()