Meprin beta Subunit/MEP1B Antibody (289731) [Alexa Fluor® 647]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 289731
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Meprin beta Subunit/MEP1B Antibody (289731) [Alexa Fluor® 647] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Meprin β Subunit/MEP1B
  Thr23-Ser293
  Accession # Q16820
• Specificity: Detects the pro form of human or mouse Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin beta Subunit/MEP1B Antibody (289731) [Alexa Fluor® 647]

• EC 3.4.24.63
• endopeptidase-2
• MEP1B
• meprin A subunit beta
• meprin A, beta
• Meprin B
• Meprin beta Subunit
• N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
• PABA peptide hydrolase
• PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only) (3). Although the two subunits share 42% amino acid (aa) sequence identity, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of human Merpin beta subunit precursor consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 61), and a mature chain (aa 62 to 701) containing catalytic (aa 62 to 259), MAM (aa 260 to 429), MATH (aa 430 to 585), EGF-like (aa 604 to 644), transmembrane (aa 653 to 673), and cytoplasmic (aa 674 to 701) domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.