![Immunocytochemistry/Immunofluorescence: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [Janelia Fluor® 549] [NBP2-34681JF549] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Janelia-Fluor-R-549-Immunocytochemistry-Immunofluorescence-NBP2-34681JF549-img0001.jpg "Immunocytochemistry/Immunofluorescence: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [Janelia Fluor® 549] [NBP2-34681JF549] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | M2-7C10 + M2-9E3 + T311 + HMB45 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Janelia Fluor 549 |
| Immunogen | Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. |
| Isotype | IgG2b Lambda/IgG2a Kappa/IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. Successful se in ICC/IF reported by customer review. |
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| Reviewed Applications |
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| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Jan Martinek |
IF | Human | 06/03/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | MLANA |
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