| Reactivity | MuSpecies Glossary |
| Applications | Flow |
| Clone | 345404 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Alexa Fluor 488 |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB3737U-100UG | |||
| Alexa Fluor 405 | FAB3737V-100UG | |||
| Alexa Fluor 594 | FAB3737T-100UG | |||
| Alexa Fluor 647 | FAB3737R-100UG | |||
| Alexa Fluor 700 | FAB3737N-100UG | |||
| Alexa Fluor 750 | FAB3737S-100UG | |||
| Unconjugated | MAB3737 | |||
| Immunogen | Y3 rat myeloid cell line transfeced with mouse Melanocortin 3R/MC3R |
| Specificity | Detects Melanocortin 3R/MC3R in direct ELISAs and Western blots. Stains mouse Melanocortin 3R/MC3R-transfected cells but not irrelevant transfectants. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | MC3R |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
| Preservative | 0.09% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Mouse Melanocortin 3R (MC3R) is a member of the melanocortin receptor family. The 323 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in the brain where, along with MC4R, it binds alpha, beta and gamma melanocyte stimulating hormones and is involved in regulating energy balance. MC3R is also expressed by macrophages and plays an anti-inflammatory role in the heart and other tissues. Of the 69 aa that are extracellular, mouse MC3R shares 91% and 78% sequence identity with rat and human MC3R, respectively.
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | FAB3737U | |
| Alexa Fluor 405 | FAB3737V | |
| Alexa Fluor 488 | FAB3737G | |
| Alexa Fluor 594 | FAB3737T | |
| Alexa Fluor 647 | FAB3737R | |
| Alexa Fluor 700 | FAB3737N | |
| Alexa Fluor 750 | FAB3737S |
Diseases for Melanocortin-3 R/MC3R Antibody (FAB3737G)Discover more about diseases related to Melanocortin-3 R/MC3R Antibody (FAB3737G).
| Pathways for Melanocortin-3 R/MC3R Antibody (FAB3737G)View related products by pathway.
|
PTMs for Melanocortin-3 R/MC3R Antibody (FAB3737G)Learn more about PTMs related to Melanocortin-3 R/MC3R Antibody (FAB3737G).
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MC3R |
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/images2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.](http://images.novusbio.com/images2/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/images2/Melanocortin-4-R-Antibody-Western-Blot-NBP2-41103-img0001.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-4 R Antibody [NBP2-41103] - human brain tissue with MC4R antibody at 2.5 ug/mL.](http://images.novusbio.com/images2/Melanocortin-4-R-Antibody-Immunohistochemistry-Paraffin-NBP2-41103-img0002.jpg)
![Immunofluorescence: Melanocortin-4 R Antibody [NBP2-41103] - Human Brain cells with MC4R antibody at 20 ug/mL.](http://images.novusbio.com/images2/Melanocortin-4-R-Antibody-Immunofluorescence-NBP2-41103-img0003.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/images2/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0002.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/images2/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0001.jpg)
![Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/images2/MSX2-Antibody-Immunohistochemistry-Paraffin-NBP1-85445-img0015.jpg)
![Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human urinary bladder shows weak nuclear positivity in urothelial cells.](http://images.novusbio.com/images2/MSX2-Antibody-Immunohistochemistry-Paraffin-NBP1-85445-img0012.jpg)
![Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/images2/MSX2-Antibody-Immunohistochemistry-Paraffin-NBP1-85445-img0013.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Mouse Lung showing detection of >150 kDa Copper Transporting ATPase 1 protein using clone S60-4. 10 ug of sample was loaded and primary antibody was used at 1:1000. Predicted/Observed Size: ~160 kDa. Other band(s): 250 kDa.](http://images.novusbio.com/images2/ATP7A-Antibody-(S60-4)-Western-Blot-NBP2-59376-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/images2/ATP7A-Antibody-(S60-4)-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0002.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Analysis of Mineralocorticoid Receptor.](http://images.novusbio.com/images2/Mineralocorticoid-R-NR3C2-Antibody-(H10E4C9F)-Western-Blot-NB300-562-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/Mineralocorticoid-R-NR3C2-Antibody-(H10E4C9F)-Immunocytochemistry-Immunofluorescence-NB300-562-img0008.jpg)
![Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/images2/Mineralocorticoid-R-NR3C2-Antibody-(H10E4C9F)-Immunohistochemistry-Paraffin-NB300-562-img0011.jpg)
![Western Blot: MC2 Receptor Antibody [NB100-93419] - (1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/MC2 Receptor Antibody-Western Blot-NB100-93419-img0002.jpg)
