Melan-A/MART-1 Antibody (SPM555) [Allophycocyanin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM555
Clonality
Monoclonal
Host
Mouse

Melan-A/MART-1 Antibody (SPM555) [Allophycocyanin] Summary

Immunogen
Recombinant hMART-1 protein
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Allophycocyanin
Format
Allophycocyanin (A=620-650, E=660-670)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Alternate Names for Melan-A/MART-1 Antibody (SPM555) [Allophycocyanin]

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • melan-A
  • Melan-A/MART-1
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP2-34797APC) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Melan-A/MART-1 Products

Melan-A/MART-1 NBP2-34797APC

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Bioinformatics Tool for Melan-A/MART-1 Antibody (NBP2-34797APC)

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Diseases for Melan-A/MART-1 Antibody (NBP2-34797APC)

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Research Areas for Melan-A/MART-1 Antibody (NBP2-34797APC)

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MLANA
Entrez

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