Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free [NBP3-24314] - Formalin-fixed, paraffin-embedded human skin stained with Melan-A/MART-1 Antibody (rMLANA/9404). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: rMLANA/9404
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human Melan-A/MART-1 protein
• Localization: Cytoplasm.
• Marker: Melanoma Marker
• Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

• Antigen LB39-AA
• Antigen SK29-AA
• Mart 1 Melan A
• MART-1
• MART1MART-1
• MelanA
• Melan-A
• melanoma antigen recognized by T-cells 1
• MLANA
• Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLANA