Melan-A/MART-1 Antibody (MLANA/788) - Azide and BSA Free

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Melan-A/MART-1 Antibody (MLANA/788) - Azide and BSA Free [NBP2-47803] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (MLANA/788) NBP2-47803F (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
MLANA/788
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Melan-A/MART-1 Antibody (MLANA/788) - Azide and BSA Free Summary

Immunogen
Recombinant human MLANA protein
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot : 0.5 - 1.0 ug/ml
  • Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml
  • CyTOF-ready
  • Immunofluorescence : 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 9p24.1 Molecular weight of antigen: 20-22kDa (doublet) Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Melan-A/MART-1 Antibody (MLANA/788) - Azide and BSA Free

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MLANA
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