Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - IHC-Prediluted

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - IHC-Prediluted [NBP2-44392] - Paraffin-embedded human melanoma. pH 9 antigen retrieval. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - IHC-Prediluted [NBP2-44392] - Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - IHC-Prediluted [NBP2-44392] - Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: IHC
• Clone: A103 + M2-7C10 + M2-9E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant full-length hMelan-A/MART-1 protein (A103; M2-7C10; M2-9E3) (Uniprot: Q16655)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Isotype: IgG1 Kappa/IgG2b Lambda/IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - IHC-Prediluted

• Antigen LB39-AA
• Antigen SK29-AA
• Mart 1 Melan A
• MART-1
• MART1MART-1
• MelanA
• Melan-A
• melanoma antigen recognized by T-cells 1
• MLANA
• Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLANA