MEF2C Antibody (681824) [Unconjugated]

MEF2C was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nucleus of myoblasts. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu
• Applications: IHC, ICC/IF
• Clone: 681824
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MEF2C Antibody (681824) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MEF2C
  Ala135-Lys239
  Accession # Q06413
• Specificity: Detects human MEF2C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SRF is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MEF2C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEF2C Antibody (681824) [Unconjugated]

• DEL5q14.3
• MEF2C
• myocyte enhancer factor 2C
• myocyte-specific enhancer factor 2C
• polypeptide C

Background

MEF2C (myocyte enhancer factor-2C) is a 51 kDa (predicted) protein that is one of four members of the MEF2/MADS (MCM1, Agamous, Deficiens, Serum response) box family of transcription factors. The 473 amino acid (aa) human MEF2C contains a MADS box for dimerization (aa 3-57), a DNA binding domain (aa 58-86), a beta domain (aa 271-278; missing in a 465 aa isoform), a transcriptional repressor (aa 368-399; missing in a 441 aa isoform) and several acetylation and ser/thr phosphorylation sites. Within aa 135-239, human MEF2C shares 95% and 98% aa identity with mouse and rat MEF2C, respectively. MEF2C plays important roles in preferential lymphoid differentiation of hematopoietic precursors, and in neuronal, cardiac and skeletal muscle morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEF2C Antibody (MAB6786)(3)

Publications using MAB6786

• JC Udeochu, S Amin, Y Huang, L Fan, ERS Torres, GK Carling, B Liu, H McGurran, G Coronas-Sa, G Kauwe, GA Mousa, MY Wong, P Ye, RK Nagiri, I Lo, J Holtzman, C Corona, A Yarahmady, MT Gill, RM Raju, SA Mok, S Gong, W Luo, M Zhao, TE Tracy, RR Ratan, LH Tsai, SC Sinha, L Gan Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience Nature Neuroscience, 2023-04-24;0(0):. 2023-04-24 [PMID: 37095396] (IHC, Mouse)
• Scarlett J. Barker, Ravikiran M. Raju, Noah E.P. Milman, Jose Davila-Velderrain, Fatima Davila-Velderrain, Fatima Gunter-Rahman et al. MEF2 is a key regulator of cognitive potential and confers resilience to neurodegeneration Science Translational Medicine 2021-11-03 [PMID: 34731014]
• Kyu Min Kim, Anshul Rana, Chan Young Park Orai1 inhibitor STIM2 beta regulates myogenesis by controlling SOCE dependent transcriptional factors Scientific Reports 2019-07-25 [PMID: 31346235] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MEF2C
• Uniprot:
  • Q06413()