MED8 Overexpression Lysate (Native)

Images

 
Western Blot: MED8 Overexpression Lysate (Adult Normal) [NBL1-12995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MED8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MED8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204026 and based on accession number NM_201542. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mediator complex subunit 8 (MED8), transcript variant 1, mRNA.
Gene
MED8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MED8

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MED8 Overexpression Lysate (Native)

  • ARC32subunit 8 homolog
  • mediator complex subunit 8mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae)

Background

This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02272
Species: Hu, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-17257
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84140
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2574
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P, IP, PLA
AF5089
Species: Hu
Applications: WB, IHC
NBP1-91810
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-38118
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF933
Species: Hu
Applications: WB, Simple Western, IHC, IP
MAB3765
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow
H00001831-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
NBP2-24569
Species: Hu, Mu, Rt, Bv, Ca, Eq, Pm
Applications: WB, Simple Western, IHC-P
NBP1-87006
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00009440-M02
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-58707
Species: Hu
Applications: WB, ICC/IF
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-58690
Species: Hu
Applications: ICC/IF
NBP2-31639
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
H00001024-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA
NBP1-85104
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for MED8 Lysate (NBL1-12995) (0)

There are no publications for MED8 Lysate (NBL1-12995).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MED8 Lysate (NBL1-12995) (0)

There are no reviews for MED8 Lysate (NBL1-12995). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MED8 Lysate (NBL1-12995) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MED8 Products

Bioinformatics Tool for MED8 Lysate (NBL1-12995)

Discover related pathways, diseases and genes to MED8 Lysate (NBL1-12995). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MED8 Lysate (NBL1-12995)

Discover more about diseases related to MED8 Lysate (NBL1-12995).
 

Pathways for MED8 Lysate (NBL1-12995)

View related products by pathway.

PTMs for MED8 Lysate (NBL1-12995)

Learn more about PTMs related to MED8 Lysate (NBL1-12995).

Blogs on MED8

There are no specific blogs for MED8, but you can read our latest blog posts.

Customers Who Bought This Also Bought

MED8 Antibody
NBP1-89014

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MED8 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MED8