MED4 Antibody [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 525

Order Details

MED4 Antibody [Janelia Fluor® 525] Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4. The immunogen is located within the last 50 amino acids of MED4. Amino Acid Squence: PGHNKEDEDDVEIMSTD
Specificity
At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED4
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MED4 Antibody [Janelia Fluor® 525]

  • Activator-recruited cofactor 36 kDa component
  • ARC36
  • DRIP36
  • DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae)
  • FLJ10956
  • HSPC126
  • HSPC126vitamin D receptor interacting protein
  • MED4
  • mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit
  • subunit 4 homolog
  • TRAP36
  • VDRIP

Background

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MED4 Products

Array NBP1-76930JF525

Research Areas for MED4 Antibody (NBP1-76930JF525)

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Bioinformatics

Gene Symbol MED4