MED4 Antibody

Images

 
Western Blot: MED4 Antibody [NBP1-76930] - Human testis tissue lysate with MED4 antibody at 0.5 ug/mL.
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-76930] - Human testis tissue with MED4 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MED4 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4. The immunogen is located within the last 50 amino acids of MED4.
Specificity
At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
MED4 Peptide (NBP1-76930PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MED4 Antibody

  • Activator-recruited cofactor 36 kDa component
  • ARC36
  • DRIP36
  • DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae)
  • FLJ10956
  • HSPC126
  • HSPC126vitamin D receptor interacting protein
  • MED4
  • mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit
  • subunit 4 homolog
  • TRAP36
  • VDRIP

Background

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment. At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MED4 Antibody (NBP1-76930)

Discover related pathways, diseases and genes to MED4 Antibody (NBP1-76930). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol MED4
Entrez
Uniprot