MED31 Antibody (2C8)

Western Blot: MED31 Antibody (2C8) [H00051003-M01] - MED31 monoclonal antibody (M01), clone 2C8 Analysis of MED31 expression in MCF-7.

Immunocytochemistry/Immunofluorescence: MED31 Antibody (2C8) [H00051003-M01] - Analysis of monoclonal antibody to MED31 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: MED31 Antibody (2C8) [H00051003-M01] - Analysis of monoclonal antibody to MED31 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Product Discontinued

MED31 Antibody (2C8) Summary

• Immunogen: MED31 (AAH12539, 1 a.a. ~ 131 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYPQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK
• Specificity: MED31 - mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (2C8)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED31
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED31 Antibody (2C8)

• CGI-125
• FLJ27436
• FLJ36714
• hSOH1
• mediator complex subunit 313110004H13Rik
• Mediator complex subunit SOH1
• mediator of RNA polymerase II transcription subunit 31
• mediator of RNA polymerase II transcription, subunit 31 homolog (S. cerevisiae)
• mediator of RNA polymerase II transcription, subunit 31 homolog
• Soh1

Background

MED31 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED31