ME1 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 488

ME1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1. The immunogen is located within the first 50 amino acids of ME1. Amino Acid Squence: MEPEAPRRRHTHQRGYL
• Specificity: ME1 antibody is predicted not to cross-react with ME2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ME1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ME1 Antibody [Alexa Fluor® 488]

• EC 1.1.1
• EC 1.1.1.40
• HUMNDME
• malate dehydrogenase
• Malic enzyme 1
• malic enzyme 1, NADP(+)-dependent, cytosolic
• malic enzyme 1, soluble
• Malic enzyme, cytoplasmic
• MES
• NADP-dependent malic enzyme
• NADP-ME
• pyruvic-malic carboxylase

Background

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ME1 Antibody (NBP2-41267AF488) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Tobias Kammann Flow Human 04/22/2024

Summary

• Application: Flow Cytometry
• Sample Tested: Blood mononuclear cells (PBMCs)
• Species: Human
• Lot: n.a.

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Intracellular staining on fixed and permeabilized human PBMCs.

Bioinformatics

• Gene Symbol: ME1