ME1 Antibody (3H5) - Azide and BSA Free

Western Blot: ME1 Antibody (3H5) [H00004199-M03] - Analysis of ME1 expression in transfected 293T cell line by ME1 monoclonal antibody (M03), clone 3H5. Lane 1: ME1 transfected lysatE (64.1 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: ME1 Antibody (3H5) [H00004199-M03] - Analysis of monoclonal antibody to ME1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.5 ug/ml

Western Blot: ME1 Antibody (3H5) [H00004199-M03] - Analysis of ME1 expression in HepG2 (Cat # L019V1).

Sandwich ELISA: ME1 Antibody (3H5) [H00004199-M03] - Detection limit for recombinant GST tagged ME1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ME1 Antibody (3H5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ME1 (AAH25246, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ
• Specificity: ME1 - malic enzyme 1, NADP(+)-dependent, cytosolic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ME1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ME1 Antibody (3H5) - Azide and BSA Free

• EC 1.1.1
• EC 1.1.1.40
• HUMNDME
• malate dehydrogenase
• Malic enzyme 1
• malic enzyme 1, NADP(+)-dependent, cytosolic
• malic enzyme 1, soluble
• Malic enzyme, cytoplasmic
• MES
• NADP-dependent malic enzyme
• NADP-ME
• pyruvic-malic carboxylase

Background

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ME1 Antibody (H00004199-M03)(2)

Publications using H00004199-M03

• Heart E, Cline GW, Collis LP et al. Role for malic enzyme, pyruvate carboxylation mitochondrial malate import in the glucose-stimulated insulin secretion . Am J Physiol Endocrinol Metab. 2009-03-17 [PMID: 19293334]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: ME1
• Entrez:
  • 4199(Human)
• OMIM:
  • 154250(Human)
• Uniprot:
  • AAH25246(Human)