ME1 Antibody (15611) - Azide and BSA Free

Western Blot: ME1 Antibody (15611) [NBP2-43669] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at a dilution of 1:1000.

Immunohistochemistry-Paraffin: ME1 Antibody (15611) [NBP2-43669] - Analysis of mouse cervix. ME1 antibody [15611] diluted at 1:500.

Immunohistochemistry-Paraffin: ME1 Antibody (15611) [NBP2-43669] - Analysis of mouse liver. ME1 antibody [15611] diluted at 1:500.

Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT15611] (NBP2-43669) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Sh
• Applications: WB, IHC
• Clone: 15611
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ME1 Antibody (15611) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ME1 Antibody (15611) - Azide and BSA Free (NBP2-43669) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.
• Predicted Species: Rat (91%), Sheep (92%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ME1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ME1 Antibody (15611) - Azide and BSA Free

• EC 1.1.1
• EC 1.1.1.40
• HUMNDME
• malate dehydrogenase
• Malic enzyme 1
• malic enzyme 1, NADP(+)-dependent, cytosolic
• malic enzyme 1, soluble
• Malic enzyme, cytoplasmic
• MES
• NADP-dependent malic enzyme
• NADP-ME
• pyruvic-malic carboxylase

Background

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ME1