Recombinant Human MDR1/ABCB1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA

Order Details

Recombinant Human MDR1/ABCB1 Protein Summary

Description
Recombinant protein with GST-tag at N-terminal corresponding to the amino acids 620 - 709 of Human ABCB1 partial ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence:GIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWP

Details of Functionality
This protein is not active and should not be used for experiments requiring activity.
Protein/Peptide Type
Partial Recombinant Protein
Gene
ABCB1
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot Western Blot
Application Notes
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.
Publications
Read Publication using H00005243-Q01.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Purity
>80%, by SDS-PAGE

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human MDR1/ABCB1 Protein

  • ABC20
  • ABCB1
  • ABCB1B
  • ATP-binding cassette sub-family B member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • CD243 antigen
  • CD243
  • CLCS
  • colchicin sensitivity
  • doxorubicin resistance
  • EC 3.6.3
  • EC 3.6.3.44
  • GP170
  • IBD13
  • MDR1
  • MDR1MGC163296
  • multidrug resistance protein 1
  • P-glycoprotein 1
  • P-gp
  • PGY1
  • PGY1P-GP

Background

ABCB1 - ATP-binding cassette, sub-family B (MDR/TAP), member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for MDR1/ABCB1 Partial Recombinant Protein (H00005243-Q01)(1)

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Video Protocols

WB Video Protocol

FAQs for MDR1/ABCB1 Partial Recombinant Protein (H00005243-Q01). (Showing 1 - 2 of 2 FAQ).

  1. I am interested in MDR1 Partial Recombinant Protein (H00005243-Q01). I've noticed that this protein is partialy recombinant. Please explain what means partialy. I would also like to know if the protein is folded correctly, since the data sheet says that it shouldn't be used for experiments requiring activity. Would you be so kind and let me know, which part of MDR1 protein is partial recombinant protein you have, is it the domain which is located on the exterior surface of plasma mambrane?
    • A parital recombinant protein is made from only a part of the DNA sequence where as a Recombinant protein is made from a full DNA sequence. This protein is produced by a Taiwanese company called Abnova. It is folded correctly however we have listed it to be biologically inactive due to many customer purchasing this from Abnova and finding that it does not function correctly therefore we have suggested not to use this for any experiments requiring activity. Amino acids 620 - 709 are in the partial recombinant protein and the domain on the exterior surface has not been specified.
  2. I have purchased H00005243-Q01, mdr1/abcb1 purified recombinant protein. The photo indicates a KDa of around 40. Do you have the exact KDa of this product? Usually the Kda of MDR1 is 170. I am using it as a positive control in western blot and I am not getting any bands.
    • I am sorry to learn that you used this partial recombinant protein in your WB as the positive control but did not yield any positive signal. I anticipate that your anti-human MDR1/ABCB1 antibody must bind to somewhere outside of 620 a.a. to 710 a.a. This partial protein contains only a part of the gene for human MDR1/ABCB1 (NP_000918, 620 a.a. - 710 a.a.) that is in-frame fused to a gene for the GST tag (~26 kDa) at its N-terminus. Therefore the apparent size for this partial protein is about 36 kDa, which is similar to that in the SDS-PAGE image posting on our webpage. According to "Uniprot Site", human MDR1/ABCB1 has two isoforms: isoform 1 has 1,280 a.a. with the calculated size of 141,479 Da, whereas isoform 2 has 1,216 a.a. (Mass in Da:134,032); and this is consistent with your information about the size of the protein. In summary this protein is apparently not suitable for your WB experiments as the positive control, unfortunately. The anti-human MDR1/ABCB1 antibody(ies) has to have the immunogen that is included in 620 a.a. to 710 a.a. in order for H00005243-Q01 to be useful as the positive control in WB.

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Bioinformatics

Gene Symbol ABCB1
Entrez