MDMX Antibody

Western Blot: MDMX Antibody [H00004194-B01P] - Analysis of MDM4 expression in transfected 293T cell line by MDM4 polyclonal antibody. Lane 1: MDM4 transfected lysate(53.9 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MDMX Antibody [H00004194-B01P] - Analysis of purified antibody to MDM4 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

MDMX Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MDM4 (NP_002384.2, 1 a.a. - 490 a.a.) full-length human protein. MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MDM4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: It has been used for WB and IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MDMX Antibody

• DKFZp781B1423
• double minute 4 homolog
• Double minute 4 protein
• double minute 4, human homolog of; p53-binding protein
• HDMX
• Mdm2-like p53-binding protein
• Mdm4 p53 binding protein homolog (mouse)
• MDM4 Regulator of P53
• MDM4
• Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
• Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
• MDM4-related protein 1
• MDMX
• MDMXMGC132766
• mouse double minute 4, human homolog of; p53-binding protein
• MRP1
• p53-binding protein Mdm4
• protein Mdm4
• Protein Mdmx

Background

Mdm4 p53 binding protein homolog (mouse). The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDMX Antibody (H00004194-B01P)(1)

Publication using H00004194-B01P

• Jacob AG. Stress-Induced Isoforms of MDM2 and MDM4 Correlate with High-Grade Disease and an Altered Splicing Network in Pediatric Rhabdomyosarcoma. Neoplasia. 2013-09-01 [PMID: 24027430]

Bioinformatics

• Gene Symbol: MDM4
• Uniprot:
  • NP_002384.2(Human)