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MDM1 Antibody (NBP1-85839) (1)
Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.
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Reviews using NBP1-85839:
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Anatoli Grechov WB
Application Western Blot Sample Tested 293T lysates Species Human Blocking
Blocking Details 5% BSA in PBS Primary Anitbody
Dilution Ratio 1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker Secondary Antibody
Secondary Description anti-rabbit IgG HRP conjugate (Jackson Labs) Secondary Manufacturer Cat# 111-005-045 Secondary Concentration 1:20000 Details
Detection Notes Probed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure
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FAQs for MDM1 Antibody (NBP1-85839). (Showing 1 -
1 of 1 FAQ).
I used an Ab NBP-1 85839 from your company in NSLC cells for western and am wondering what the expected size of this protein should be.
The MDM1 protein has an expected molecular weight of 80kDa. There are four isoforms to this protein and our product NBP1-85839 is capable of detecting isoforms 1 and 4 (80 and 76kDa respectively).
uniprot site In western blot, our lab has detected bands from 70-78kDa. Since there is a phophoserine residue at amino acid 83, it is possible to detect bands up to 80-85kDa.
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