MDC1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: MDC1 Antibody [NBP1-84860] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.
Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MDC1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:REAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAG
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MDC1 Protein (NBP1-84860PEP)
Publications
Read Publication using
NBP1-84860 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23833192).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MDC1 Antibody

  • DKFZp781A0122
  • Em:AB023051.5
  • KIAA0170MGC166888
  • MDC1
  • mediator of DNA damage checkpoint 1
  • mediator of DNA damage checkpoint protein 1
  • mediator of DNA-damage checkpoint 1
  • NFBD1
  • NFBD1homologue to Drosophila photoreceptor protein calphotin
  • Nuclear factor with BRCT domains 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDC1 Antibody (NBP1-84860)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for MDC1 Antibody (NBP1-84860) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MDC1 Products

MDC1 NBP1-84860

Bioinformatics Tool for MDC1 Antibody (NBP1-84860)

Discover related pathways, diseases and genes to MDC1 Antibody (NBP1-84860). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MDC1 Antibody (NBP1-84860)

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Research Areas for MDC1 Antibody (NBP1-84860)

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Blogs on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol MDC1
Entrez
Uniprot