| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12890-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6497-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56657SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84860-25ul | |||
![Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0018.jpg "Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NB100-395] - PARP activity affects 53BP1 recruitment to low-input power damage. Immunofluorescent localization of MDC1 and 53BP1 in HeLa cells irradiated with 15% (indicated by white arrowheads) and/or 30% (indicated by yellow arrowheads) at 30 min p.i. N = 10 with consistent results. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv976), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-395-img0020.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0016.jpg)
![Immunoprecipitation: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ug per reaction. MDC1 was also immunoprecipitated by two other rabbit anti-MDC1 antibodies. For blotting immunoprecipitated MDC1, NB100-395 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunoprecipitation-NB100-395-img0019.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - BRUCE UBC domain is required for NBS1 repair foci formation.U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants as indicated to the left were treated with DOX to knockdown endogenous BRUCE. After irradiation, cells were fixed & immunofluorescence stained for NBS1 (A) & quantified for cells with more than five nuclear foci (B). Bars, 10 μm. Error bars represent standard deviation from a triplicate of a representative experiment. DOX treated shBRUCE-U2OS cells were irradiated & the cell lysates subject to immunoblotting with the indicated antibodies showing the protein levels of ATM, MDC1 & NBS1 are not reduced post BRUCE knockdown (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26683461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-395_rabbit-polyclonal-mdc1-antibody-31020241534531.jpg)
![Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-397-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDC1 Antibody [NB100-397] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NB100-397-img0002.jpg)
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0004.jpg "Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890] - Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Immunocytochemistry-Immunofluorescence-NBP2-12890-img0003.jpg)
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0001.jpg)
![Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-56657-img0002.jpg "Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84860-img0006.jpg "Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.")
![Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-84860-img0007.jpg)
![Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-84860-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining in human testis and liver tissues using anti-MDC1 antibody. Corresponding MDC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-84860-img0008.jpg)
![Immunohistochemistry;MDC1 Antibody[NBP3-29608]Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29609_rabbit-mdc1-pab-78202410153551.jpg "Immunohistochemistry;MDC1 Antibody[NBP3-29608]Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 used at a dilution of 1:1,000 . Detection: DAB")