MDC1 Antibody (398636)

Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Human MDC1 Monoclonal Antibody (Catalog # MAB6497) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MDC1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 398636
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

MDC1 Antibody (398636) Summary

• Immunogen:

  E.coli-derived recombinant human MDC1

  
  Met1-Glu335
  Accession # Q14676
• Specificity: Detects human MDC1 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MDC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MDC1 Antibody (398636)

• DKFZp781A0122
• Em:AB023051.5
• KIAA0170MGC166888
• MDC1
• mediator of DNA damage checkpoint 1
• mediator of DNA damage checkpoint protein 1
• mediator of DNA-damage checkpoint 1
• NFBD1
• NFBD1homologue to Drosophila photoreceptor protein calphotin
• Nuclear factor with BRCT domains 1

Background

Mediator of DNA damage checkpoint protein 1 (MDC1), also known as nuclear factor with BRCT domains protein 1 (NFBD1), is a 250 kDa nuclear protein that is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage.  Human MDC1 is 2089 amino acids (aa) in length and contains a forkhead-associated motif at its N terminus, two BRCT motifs at its C terminus, and 13 internal repetitions of a 41 aa sequence enriched with seine, threonine, and proline residues.  Splicing variants produce five isoforms of MDC1.  Human MDC1 shares 49% aa sequence identity with mouse MDC1.  MDC1 is highly expressed in testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDC1 Antibody (MAB6497)(1)

Publication using MAB6497

• Hang Q, Zeng L, Wang L et al. Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance Nature Communications 2021-06-29 [PMID: 34188037]

Bioinformatics

• Gene Symbol: MDC1
• Uniprot:
  • Q14676()