MDC1 Antibody (398636) [Alexa Fluor® 405]

• Applications: WB
• Clone: 398636
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

MDC1 Antibody (398636) [Alexa Fluor® 405] Summary

• Immunogen:

  E.coli-derived recombinant human MDC1

  
  Met1-Glu335
  Accession # Q14676
• Specificity: Detects human MDC1 in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Mediator of DNA damage checkpoint protein 1 (MDC1), also known as nuclear factor with BRCT domains protein 1 (NFBD1), is a 250 kDa nuclear protein that is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage.  Human MDC1 is 2089 amino acids (aa) in length and contains a forkhead-associated motif at its N terminus, two BRCT motifs at its C terminus, and 13 internal repetitions of a 41 aa sequence enriched with seine, threonine, and proline residues.  Splicing variants produce five isoforms of MDC1.  Human MDC1 shares 49% aa sequence identity with mouse MDC1.  MDC1 is highly expressed in testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.