MD-1/LY86 Antibody (142004)

• Reactivity: Mu
• Applications: WB
• Clone: 142004
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

MD-1/LY86 Antibody (142004) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MD-1
  Asp20-Ser162
  Accession # O88188
• Specificity: Detects mouse MD-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant human (rh) MD-1. In Western blots, this antibody does not cross-react with rhMD-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: LY86
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MD-1/LY86 Antibody (142004)

• LY86
• Ly-86
• lymphocyte antigen 86
• MD1
• MD-1
• MMD-1
• Protein MD-1
• RP105-associated

Background

MD-1 is a secreted glycoprotein that was originally identified as a v-myb-regulated gene from avian myeloleukemia virus-transformed chicken myeloblasts (1). The mouse homologue of chicken MD-1 (also known as lymphocyte antigen 86) was subsequently discovered as a molecule that is associated with RP105, a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, RP105 has a short cytoplasmic tail and lacks the Toll-IL-1 R (TIR) domain that defines the IL-1 R/TLR superfamily (2 - 4). RP105 plays an important role in B-cell activation by bacterial lipopolysaccharide (LPS). It is expressed primarily on mature B cells, dendritic cells and macrophages (4). Mouse MD-1 cDNA encodes a 162 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% aa sequence identity with chicken and human MD-1 respectively (2, 3). MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney. MD-1 is required for efficient RP105 cell surface expression and function (2 - 5). Cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B-cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation and upregulate B7.2, a co-stimulatory molecule (5, 6). Since MD-1 is also expressed in liver and brain where RP105 is absent, it has been speculated that MD-1 can also be associated with other LRR-containing molecules, or have additional functions outside the immune system (6).

1. Burk, O. and K. Klempnauer (1991) EMBO J. 10:3713.
2. Miyake, K. et al. (1998) J. Immun. 161:1348.
3. Miura, Y. et al. (1998) Blood 92:2815.
4. Miyake, K. et al. (1995) J. Immunol. 154:3333.
5. Nagai, Y. et al. (2002) Blood 99:1699.
6. Ogata, H. et al. (2000) J. Exp. Med. 192:23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY86
• Uniprot:
  • O88188()