MCT1/SLC16A1 Overexpression Lysate

Images

 
Western Blot: Monocarboxylic acid transporter 1 Overexpression Lysate (Adult Normal) [NBP2-11461] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monocarboxylic ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MCT1/SLC16A1 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001159968. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Monocarboxylic acid transporter 1, mRNA.
Gene
SLC16A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MCT1/SLC16A1 Overexpression Lysate

  • FLJ36745
  • HHF7
  • MCT 1
  • MCT
  • MCT1
  • MCT1MGC44475
  • member 1
  • monocarboxylate transporter 1
  • SLC16A1
  • Solute carrier family 16 member 1
  • solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Other Available Formats

NBP2-11461

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Blogs on MCT1/SLC16A1.

Monocarboxylate Transporter 1 (MCT1) - a novel oncogene
MCT1 is a proton-linked transport carrier that catalyzes the movement of short chain monocarboxylates (branched-chain oxo acids derived from leucine, valine, and isoleucine) across both plasma and inner mitochondrial membranes. In particular, subst...  Read full blog post.

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Bioinformatics

Gene Symbol SLC16A1