MCPH1 Overexpression Lysate

Western Blot: Microcephalin 1 Overexpression Lysate (Adult Normal) [NBL1-12961] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Microcephalin 1.

• Reactivity: Hu
• Applications: WB

MCPH1 Overexpression Lysate Summary

• Description:

  MCPH1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC224920
  
  Accession#: NM_024596
  
  Protein Tag: C-MYC/DDK
• Gene: MCPH1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 92.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MCPH1 Overexpression Lysate

• BRCT-repeat inhibitor of TERT expression 1
• BRIT1
• FLJ12847
• MCPH1
• MCT
• microcephalin 1
• Microcephalin
• microcephaly, primary autosomal recessive 1

Background

MCPH-1 was identified in a screen for negative regulators of hTERT (human telomerase) expression. It has been implicated in chromosome condensation and DNA damage induced cellular responses. It may play a role in neurogenesis and regulation of the size of the cerebral cortex. Defects in MCPH1 are the cause of primary microcephaly 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MCPH1