MCPH1 Antibody [Alexa Fluor® 350]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

MCPH1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human MCPH1
  Met1-Lys250
  Accession # Q8NEM0
• Specificity: Detects human, mouse and rat MCPH1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

MCPH1 (microcephalin 1), originally identified as an inhibitor of hTERT expression, has been implicated in DNA damage response. MCPH1 contains one N-terminal and two C-terminal BRCT domains. BRCT domains are found predominantly in cell cycle proteins responsive to DNA damage. MCPH1 forms irradiation-induced nuclear foci (IRIF) that colocalize with NBS1, 53BP1, MDC1, and ATM and are abolished with MCPH1 specific siRNA. MCPH1 also regulates the ATR pathway. It colocalizes with ATR and RPA and is required for the phosphorylation of RPA and Rad17. Defects in MCPH1 are the cause of primary microcephaly 1, an autosomal recessive neurodevelopmental disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.