Recombinant Human MCM7 His Protein

SDS-Page: MCM7 Recombinant Protein [NBP2-51540] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human MCM7 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-414 of Human MCM7
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVVATYT CDQCGAETYQ PIQSPTFMPL IMCPSQECQT NRSGGRLYLQ TRGSRFIKFQ EMKMQEHSDQ VPVGNIPRSI TVLVEGENTR IAQPGDHVSV TGIFLPILRT GFRQVVQGLL SETYLEAHRI VKMNKSEDDE SGAGELTREE LRQIAEEDFY EKLAASIAPE IYGHEDVKKA LLLLLVGGVD QSPRGMKIRG NINICLMGDP GVAKSQLLSY IDRLAPRSQY TTGRGSSGVG LTAAVLRDSV SGELTLEGGA LVLADQGVCC IDEFDKMAEA DRTAIHEVME QQTISIAKAG ILTTLNARCS ILAAANPAYG RYNPRRSLEQ NIQLPAALLS RFDLLWLIQD RPDRDNDLRL AQHITYVHQH SRQPPSQFEP LDMKLMRRYI AMCREKQPMV PESLADYITA AYVEMRR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MCM7
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 48.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human MCM7 His Protein

• CDC47 homolog
• CDC47
• CDC47P85MCM
• DNA replication licensing factor MCM7
• EC 3.6.4.12
• homolog of S. cerevisiae Cdc47
• MCM2
• MCM2PNAS146
• MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
• MCM7
• minichromosome maintenance complex component 7
• minichromosome maintenance deficient (S. cerevisiae) 7
• minichromosome maintenance deficient 7
• P1.1-MCM3
• P1CDC47
• P85MCM
• PNAS146
• PPP1R104
• Protein Phosphatase 1

Background

The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MCM7