| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P |
| Clone | MCM7/1469 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 680 |
| Immunogen | Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary) |
| Localization | Nuclear |
| Marker | Proliferation Marker |
| Specificity | The specificity of this monoclonal antibody to human MCM7 was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by theMCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MCM7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![MCM7 Antibody (MCM7/1469) [DyLight 680]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "MCM7 Antibody (MCM7/1469) [DyLight 680]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-59614AF350 | |
| Alexa Fluor 405 | NBP2-59614AF405 | |
| Alexa Fluor 488 | NBP2-59614AF488 | |
| Alexa Fluor 532 | NBP2-59614AF532 | |
| Alexa Fluor 594 | NBP2-59614AF594 | |
| Alexa Fluor 647 | NBP2-59614AF647 | |
| Alexa Fluor 700 | NBP2-59614AF700 | |
| Alexa Fluor 750 | NBP2-59614AF750 | |
| Allophycocyanin | NBP2-59614APC | |
| Biotin | NBP2-59614B | |
| DyLight 350 | NBP2-59614UV | |
| DyLight 405 | NBP2-59614V | |
| DyLight 488 | NBP2-59614G | |
| DyLight 550 | NBP2-59614R | |
| DyLight 594 | NBP2-59614DL594 | |
| DyLight 650 | NBP2-59614C | |
| DyLight 680 | NBP2-59614FR | |
| DyLight 755 | NBP2-59614IR | |
| FITC | NBP2-59614F | |
| HRP | NBP2-59614H | |
| Janelia Fluor 549 | NBP2-59614JF549 | |
| Janelia Fluor 646 | NBP2-59614JF646 | |
| PE | NBP2-59614PE | |
| PerCP | NBP2-59614PCP |
Diseases for MCM7 Antibody (NBP2-59614FR)Discover more about diseases related to MCM7 Antibody (NBP2-59614FR).
| Pathways for MCM7 Antibody (NBP2-59614FR)View related products by pathway.
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PTMs for MCM7 Antibody (NBP2-59614FR)Learn more about PTMs related to MCM7 Antibody (NBP2-59614FR).
| Research Areas for MCM7 Antibody (NBP2-59614FR)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MCM7 |
![Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/PIK3CA-Antibody-Western-Blot-NBP2-19804-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/PIK3CA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19804-img0009.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3 kinase p110 alpha antibody.](http://images.novusbio.com/images2/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NBP2-19804-img0004.jpg)
![Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/images2/MCM5-Antibody-Western-Blot-NB100-79778-img0012.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/MCM5-Antibody-Immunohistochemistry-NB100-79778-img0010.jpg)
![Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.](http://images.novusbio.com/images2/MCM4-Antibody-Western-Blot-NBP1-33105-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MCM4 Antibody [NBP1-33105] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/MCM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33105-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/images2/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-33105-img0004.jpg)
![Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/MCM6-Antibody-Western-Blot-NBP1-82642-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MCM6 Antibody [NBP1-82642] - Staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/images2/MCM6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82642-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining in human lymph node and liver tissues using anti-MCM6 antibody. Corresponding MCM6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MCM6-Antibody-Immunohistochemistry-Paraffin-NBP1-82642-img0009.jpg)
![Western Blot: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell (2) and Hela (3)cell lysates using anti-MCM3 antibody at 1/500 dilution.](http://images.novusbio.com/images2/MCM3 Antibody (JB99-35)-Western Blot-NBP2-75569-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (JB99-35) [NBP2-75569] - Staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/MCM3-Antibody-(JB99-35)-Immunocytochemistry-Immunofluorescence-NBP2-75569-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of paraffin-embedded rat testis tissue using anti-MCM3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/MCM3-Antibody-(JB99-35)-Immunohistochemistry-Paraffin-NBP2-75569-img0008.jpg)
![Western Blot: Cdc6 Antibody [NBP2-15837] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Cdc6-Antibody-Western-Blot-NBP2-15837-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Cdc6 Antibody [NBP2-15837] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC6 protein stained by CDC6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Cdc6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15837-img0006.jpg)
![Immunohistochemistry-Paraffin: Cdc6 Antibody [NBP2-15837] - Human oral carcinoma. CDC6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/images2/Cdc6-Antibody-Immunohistochemistry-Paraffin-NBP2-15837-img0007.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306. Antibody at 1:2000. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Western-Blot-NB500-106-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY licence.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunocytochemistry-Immunofluorescence-NB500-106-img0013.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunohistochemistry-NB500-106-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CDC45L Antibody (JJ091-04) [NBP2-67897] - Staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/CDC45L-Antibody-(JJ091-04)-Immunocytochemistry-Immunofluorescence-NBP2-67897-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/CDC45L-Antibody-(JJ091-04)-Immunohistochemistry-Paraffin-NBP2-67897-img0006.jpg)
![Flow Cytometry: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/CDC45L-Antibody-(JJ091-04)-Flow-Cytometry-NBP2-67897-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CDC7 Antibody [NBP2-32708] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytokinetic bridge & mitotic spindle. Antibody stainng is shown in green.](http://images.novusbio.com/images2/CDC7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32708-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/images2/CDC7-Antibody-Immunohistochemistry-Paraffin-NBP2-32708-img0010.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human stomach, upper shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/CDC7-Antibody-Immunohistochemistry-Paraffin-NBP2-32708-img0002.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](http://images.novusbio.com/images2/p21-CIP1-CDKN1A-Antibody-(WA-1-(HJ21))-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg)
![Western Blot: CDT1 Antibody [NB100-2567] - Human B cells. KSHV infection protects CDT1 from hypoxia mediated degradation. Mock or KSHV infected cells were grown under normoxic or hypoxic condition followed by measuring CDT1 levels. Western blot image submitted by a verified customer review.](http://images.novusbio.com/images2/CDT1-Antibody-Western-Blot-NB100-2567-img0005.jpg)
![Immunohistochemistry-Paraffin: CDT1 Antibody [NB100-2567] - Human islet cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/mL).](http://images.novusbio.com/images2/CDT1-Antibody-Immunohistochemistry-Paraffin-NB100-2567-img0004.jpg)
![Western Blot: CDT1 Antibody [NB100-2567] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti- CDT1 antibody (NB100-2567) used for WB at 1 ug/mL (A and B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/images2/CDT1-Antibody-Western-Blot-NB100-2567-img0003.jpg)
![Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/Geminin Antibody (1A8)-Western Blot-H00051053-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/Geminin-Antibody-(1A8)-Immunocytochemistry-Immunofluorescence-H00051053-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.](http://images.novusbio.com/images2/Geminin-Antibody-(1A8)-Immunohistochemistry-Paraffin-H00051053-M01-img0010.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/images/Cdk2 Antibody (2D9)-Western Blot-NBP2-00776-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/images/Cdk2 Antibody (2D9)-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0007.jpg)
![Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/images/Cdk2 Antibody (2D9)-Immunohistochemistry-Paraffin-NBP2-00776-img0006.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.](http://images.novusbio.com/images2/p16INK4a-CDKN2A-Antibody-Western-Blot-NB200-106-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0004.jpg)
![Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.](http://images.novusbio.com/images2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0006.jpg)
![Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/DBF4 Antibody (6G9)-Western Blot-H00010926-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/DBF4-Antibody-(6G9)-Immunocytochemistry-Immunofluorescence-H00010926-M01-img0004.jpg)
![Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]](http://images.novusbio.com/images2/DBF4-Antibody-(6G9)-Immunohistochemistry-Paraffin-H00010926-M01-img0001.jpg)
![Western Blot: MCM10 Antibody [NBP1-92102] - Analysis in control (vector only transfected HEK293T lysate) and MCM10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/MCM10-Antibody-Western-Blot-NBP1-92102-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/images2/MCM10-Antibody-Immunohistochemistry-Paraffin-NBP1-92102-img0007.jpg)
![Immunohistochemistry-Paraffin: MCM10 Antibody [NBP1-92102] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/images2/MCM10-Antibody-Immunohistochemistry-Paraffin-NBP1-92102-img0003.jpg)