MCM2 [p Ser108] Antibody

Images

 
Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with etoposide (100 uM, 2h). Antibodies: MCM2 was ...read more
Immunohistochemistry: MCM2 [p Ser108] Antibody [NB100-639] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) ...read more
Flow Cytometry: MCM2 [p Ser108] Antibody [NB100-639] - Flow cytometric analysis of phospho-MCM2 (pS108). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml ...read more
Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Detection of Human phospho MCM2 (Ser108) by Western Blot. Samples: Whole cell lysate from HeLa cells that were untreated or exposed to 8 Gy IR for 1 h or 50 J/m2 for ...read more
Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Whole cell lysate (15 or 50 ug) from 293T cells or mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with etoposide (100 uM, 2h). Antibody ...read more
Proximity Ligation Assay: MCM2 [p Ser108] Antibody [NB100-639] - Secondary-conjugate Duolink II PLA in Hela cells, goat anti-human MCM7 (NB100-252) and rabbit anti-human phospho-MCM2 (s108) (NB100-639). Image merged ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

MCM2 [p Ser108] Antibody Summary

Immunogen
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 108 according to the numbering given in entry NP_0045117.2 (GeneID 4171).
Modification
p Ser108
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
MCM2 Lysate (NBL1-12950)
Publications
Read Publications using NB100-639.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM2 [p Ser108] Antibody

  • BM28
  • BM28MGC10606
  • CCNL 1
  • CCNL1
  • cdc19
  • CDCL1
  • CDCL1mitotin
  • D3S3194
  • EC 3.6.4.12
  • KIAA0030MITOTIN
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
  • MCM2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • minichromosome maintenance deficient 2 (mitotin)
  • Mitotin

Background

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCM2 Antibody (NB100-639)(6)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MCM2
Entrez