MCM2 [p Ser41] Antibody

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Biological Strategies: Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody ...read more
Immunohistochemistry: MCM2 [p Ser41] Antibody [NB100-2569] - Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). ...read more
Flow Cytometry: MCM2 [p Ser41] Antibody [NB100-2569] - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

MCM2 [p Ser41] Antibody Summary

Immunogen
Immunogen was a synthetic di-phosphorylated peptide, which represented a portion of human minichromosomal maintenance deficient 2 surrounding Serines 40 and 41 according to the numbering given in entry NP_004517.2 (GeneID 4171).
Modification
p Ser41
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry 0.1 ug per 1 - 10^6 cells
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
MCM2 Overexpression Lysate
Publications
Read Publications using
NB100-2569 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM2 [p Ser41] Antibody

  • BM28
  • BM28MGC10606
  • CCNL 1
  • CCNL1
  • cdc19
  • CDCL1
  • CDCL1mitotin
  • D3S3194
  • EC 3.6.4.12
  • KIAA0030MITOTIN
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
  • MCM2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • minichromosome maintenance deficient 2 (mitotin)
  • Mitotin

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCM2 Antibody (NB100-2569)(3)

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MCM2
Entrez
Uniprot