MCM2 [p Ser53] Antibody

Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunohistochemistry: MCM2 [p Ser53] Antibody [NB100-2339] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 phospho MCM2 (S53) fs20 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MCM2 [p Ser53] Antibody Summary

• Description: Novus Biologicals Rabbit MCM2 [p Ser53] Antibody (NB100-2339) is a polyclonal antibody validated for use in IHC and WB. Anti-MCM2 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: mmunogen was a synthetic phosphorylated peptide, which represented a portion of human minichromosomal maintenance deficient 2 surrounding Serine 53 according to the numbering given in entry NP_004517.2 (GeneID 4171).
• Modification: p Ser53
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MCM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1:1000-1:2500
• Control:

  TCF-12/HTF4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MCM2 [p Ser53] Antibody

• BM28
• BM28MGC10606
• CCNL 1
• CCNL1
• cdc19
• CDCL1
• CDCL1mitotin
• D3S3194
• EC 3.6.4.12
• KIAA0030MITOTIN
• MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• MCM2
• minichromosome maintenance complex component 2
• minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
• minichromosome maintenance deficient 2 (mitotin)
• Mitotin

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM2 Antibody (NB100-2339)(5)

Publications using NB100-2339

• Kitamura R, Fukatsu R, Kakusho N et al. Molecular mechanism of activation of human Cdc7 kinase: bipartite interaction with Dbf4/activator of S phase kinase (ASK) activation subunit stimulates ATP binding and substrate recognition. J Biol Chem. 2011-07-01 [PMID: 21536671]
• Rodriguez-Acebes S, Proctor I, Loddo M et al. Targeting DNA replication before it starts: Cdc7 as a therapeutic target in p53-mutant breast cancers. Am J Pathol 2010-10-01 [PMID: 20724597]
• Hughes S, Jenkins V, Dar MJ et al. Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem 2010-01-01 [PMID: 19864417]
• Cremona CA, Lloyd AC. Loss of anchorage in checkpoint-deficient cells increases genomic instability and promotes oncogenic transformation. J Cell Sci 2009-09-01 [PMID: 19690052]
• Kulkarni AA, Kingsbury SR, Tudzarova S et al. Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma. Clin Cancer Res 2009-04-01 [PMID: 19318489]

Bioinformatics

• Gene Symbol: MCM2
• Entrez:
  • 4171(Human)
• Uniprot:
  • P49736()