MCM2 Antibody (S02-0D3)

Images

 
Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.
Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunohistochemistry of MCM2 in paraffin-embedded Human tonsil using NBP3-19887 at dilution 1/50
Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunocytochemistry of MCM2 (green) in hela using NBP3-19887 at dilution 1/5, and DAPI (blue)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S02-0D3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

MCM2 Antibody (S02-0D3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human MCM2 (Uniprot # P49736)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MCM2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for MCM2 Antibody (S02-0D3)

  • BM28
  • BM28MGC10606
  • CCNL 1
  • CCNL1
  • cdc19
  • CDCL1
  • CDCL1mitotin
  • D3S3194
  • EC 3.6.4.12
  • KIAA0030MITOTIN
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
  • MCM2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • minichromosome maintenance deficient 2 (mitotin)
  • Mitotin

Background

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCM2