MCG10 Overexpression Lysate (Native)

Images

 
Western Blot: MCG10 Overexpression Lysate (Adult Normal) [NBL1-14145] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCG10.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MCG10 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200749 and based on accession number NM_033008. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens poly(rC) binding protein 4 (PCBP4), transcript variant 3, mRNA.
Gene
PCBP4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MCG10

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MCG10 Overexpression Lysate (Native)

  • alphaCP-4 protein
  • alpha-CP4
  • CBP
  • LIP4
  • LYST-interacting protein
  • MCG10
  • poly(rC) binding protein 4
  • poly(rC)-binding protein 4
  • RNA binding protein MCG10

Background

This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined. [provided by RefSeq]. Transcript Variant: This variant (3) has an additional segment in its 5' UTR with multiple coding region differences when compared to variant 1. The resulting protein (isoform c) has a distinct N-terminus and lacks an internal segment of residues. Isoform c is also encoded by transcript variant 4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PCBP4