MBTPS1 Overexpression Lysate (Native)

Images

 
Western Blot: MBTPS1 Overexpression Lysate (Adult Normal) [NBL1-12936] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MBTPS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MBTPS1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC212265 and based on accession number NM_003791. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens membrane-bound transcription factor peptidase, site 1 (MBTPS1), mRNA.
Gene
MBTPS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
115.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MBTPS1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MBTPS1 Overexpression Lysate (Native)

  • EC 3.4.21.112
  • Endopeptidase S1P
  • KIAA0091MGC138712
  • membrane-bound transcription factor peptidase, site 1
  • membrane-bound transcription factor protease, site 1
  • PCSK8
  • S1PMGC138711
  • site-1 protease
  • SKI1
  • SKI-1membrane-bound transcription factor site-1 protease
  • subtilisin/kexin isozyme-1
  • Subtilisin/kexin-isozyme 1

Background

The encoded protein has a central role in the regulation of lipid metabolism in cells. It is a sterol-regulated subtilisin-like serine protease that cleaves ER membrane-bound sterol regulatory element-binding proteins (SREBPs), a reaction that initiates the two-step proteolytic process by which transcriptionally active fragments of SREBPs are released from the membrane for translocation to the nucleus. The gene product is an integral membrane ER protein, with the bulk located in the ER lumen. It is synthesized as an inactive preproprotein that is self-activated by an intramolecular cleavage that generates the mature protein. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MBTPS1 Lysate (NBL1-12936) (0)

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Bioinformatics

Gene Symbol MBTPS1