MBP Antibody - BSA Free

Western Blot: MBP Antibody [NBP2-29656] - rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Rat mixed neuron/glial cultures stained with chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunostaining of cultured neonatal rat neurons and glia stained with anti-myelin basic protein antibody (red, 1:500). The blue stain is to identify nuclear DNA. Note the antibody stains the myelin basic protein located in oligodendrocyte and myelin sheathes around axons in the cells.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

MBP Antibody - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Three peptide sequences conserved in higher vertebrate MBP proteins. Accession # P02688
• Marker: Oligodendrocyte Marker, Myelin Marker
• Specificity: Specific for endogenous levels of the ~18 kDa & ~22 kDa myelin basic protein.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: MBP
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:500 - 1:5000
  • Western Blot 1:5000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is expected that the antibody will react in other mammalian tissues.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 10mM Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for MBP Antibody - BSA Free

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBP
• Entrez:
  • 24547(Rat)
• Uniprot:
  • P02688(Rat)