MBP Antibody

Immunohistochemistry-Paraffin: MBP Antibody [NB100-872] - Formalin fixed paraffin embedded brain .

Immunohistochemistry-Paraffin: MBP Antibody [NB100-872] - Formalin fixed paraffin embedded human brain stained with MBP antibody.

Immunohistochemistry-Paraffin: MBP Antibody [NB100-872] - Staining of brain medula.

Product Discontinued

MBP Antibody Summary

• Immunogen: Human myelin basic protein isolated from normal human brain.
• Localization: Cytoplasmic
• Marker: Oligodendrocyte Marker, Myelin Marker
• Specificity: This reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This also stains peripheral nerves.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBP
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This antibody may be diluted to a titer of 1:50-1:100 in an ABC allmethod. We suggest an incubation period of 30 minutes at room temperature. Suitable for formalin/paraffin tissues. Depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the end user. IHC-Fr: 1/1000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Whole antisera
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for MBP Antibody

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (NB100-872)(1)

Publication using NB100-872

• Miller NR, Johnson MA, Nolan T et al. A Single Intravitreal Injection of Ranibizumab Provides No Neuroprotection in a Nonhuman Primate Model of Moderate-to-Severe Nonarteritic Anterior Ischemic Optic Neuropathy. Invest Ophthalmol Vis Sci 2015-12-01 [PMID: 26624498]

FAQs for MBP Antibody (NB100-872). (Showing 1 - 1 of 1 FAQ).

1. Is your antibody NB100-872 indicated for IHC ion mouse tissue? 
   • NB100-872 has not yet been validated in mouse. It has been validated in frozen tissue sections on human and rat. We have not yet tested it in mouse and do not have any further information available.

Bioinformatics

• Gene Symbol: MBP
• Entrez:
  • 4155(Human)
  • 24547(Rat)