MBP Antibody (BDI221)

Immunohistochemistry-Paraffin: MBP Antibody (BDI221) [NBP1-40052] - Analysis of anti-Myelin Basic Protein antibody with human brain, oligodendrocytes at concentration 5 ug/ml.

Product Discontinued

MBP Antibody (BDI221) Summary

• Immunogen: Purified human MBP.
• Marker: Oligodendrocyte Marker, Myelin Marker
• Specificity: Recognizes residues 130-136 in human MBP (21.5kD and 18.5kD molecular forms).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 10 - 20 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MBP Antibody (BDI221)

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (NBP1-40052)(1)

Publication using NBP1-40052

• Munesue Y, Ageyama N, Kimura N et al. Cynomolgus macaque model of neuronal ceroid lipofuscinosis type 2 disease Experimental neurology 2023-03-12 [PMID: 36918063] (IHC-P, Rhesus Macaque)
  
  Details:
  1:500 dilution

Bioinformatics

• Gene Symbol: MBP
• Entrez:
  • 4155(Human)
• Uniprot:
  • P02686()