MBP Antibody (7G7) [PE]

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq
• Applications: WB, ICC/IF, IHC, IP
• Clone: 7G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

MBP Antibody (7G7) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Purified myelin basic protein isolated from bovine brain, epitope is in peptide TPPPSQGKG, amino acids 125-133 of the human 21.5kDa sequence.
• Marker: Oligodendrocyte Marker, Myelin Marker
• Specificity: The MBP Antibody (7G7) antibody binds all four of the CNS MBP isoforms, so that the epitope for the antibody is located in the core shared by all four gene products.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBP
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: This Myelin Basic Protein (7G7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In WB bands can be seen at ~18 and 21.5 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
  
  Recommended applications are based on validated applications from the unconjugated base product NBP1-05203. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This peptide is invariant in rat, mouse, cow and many other species, so this antibody will have wide applicability. TMW ranges are 14 - 21.5 kDa (rodent) and 17.2 - 21.5 kDa (human).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Ascites

Alternate Names for MBP Antibody (7G7) [PE]

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBP